PRODUCTION BACTERIOCIN BY LACTOBACILLUS SSP AND ANTIBACTERIAL ACTIVITY AGAINST STAPHYLOCOCCUS AUREUS AND BIOFILM FORMATION

Authors

  • Mohsen Hashem Risan

Abstract

Antimicrobial activity of Lactobacillus spp. especially (L. planetarium and L. acidophilus) against S. aureus were tested using agar-plug, agar well diffusion methods to select the best isolate that could inhibit the growth of multidrug resistance isolates. Further identification for the presence of bacteriocin was done using ELISA kit. Results showed that Lactobacillus spp isolates were bacteriocin producers with different degrees and that L. planetarium (L7) was the most efficient in bacteriocin production. Therefore, L. planetarium (L7) was selected for purification using 70% saturated ammonium sulfate and gel chromatography. The effect of purified bacteriocin was tested on 16 bacterial isolates using micro-titer plate method and well diffusion method. The results showed the ability of the bacteriocin to inhibit bacteria only at concentrations 1866U/ml (50%), 3732U/ml (100%) with a diameter of inhibition zones ranges between (11-23 mm) respectively. The anti-biofilm activity of purified bacteriocin at concentration 100% was investigated and the results showed that biofilm formation was reduced by 100% in the presence of bacteriocin.

Author Biography

Mohsen Hashem Risan

The author suggests the existence of the undoubted influence of the background culture and mass culture on the effectiveness of rehabilitation processes.  The tasks undertaken by social rehabilitation pedagogues and people involved in the re-use of different types of cultural engineering should take into account the above influence and the image of contemporary Western culture affected by the crisis of values.  Presented concepts of resocialization through cultural activity and artistic creation are a testimony of truly humanistic search for remedial shaping of people who are socially maladjusted and show an important current in contemporary resocialization.

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Published

2022-12-05

How to Cite

Risan, M. H. (2022). PRODUCTION BACTERIOCIN BY LACTOBACILLUS SSP AND ANTIBACTERIAL ACTIVITY AGAINST STAPHYLOCOCCUS AUREUS AND BIOFILM FORMATION. TMP Universal Journal of Research and Review Archives, 1(2), 102–114. Retrieved from http://tmp.twistingmemoirs.com/index.php/ujrra/article/view/31

Issue

Vol. 1 No. 2 (2022): TMP Universal Journal of Research and Review Archives

Section

Original Article

License

Copyright (c) 2022 Mohsen Hashem Risan

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an Open Access article distributed under the terms of the Attribution 4.0 International License [CC BY 4.0], which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator.