Introduction: Noise is a significant environmental pollutant that impacts quality of life, particularly in environments such as hospitals where peace is crucial due to the nature of the work. This research aimed to examine the combined effects of hunger and noise exposure, and their influence on the cortisol levels of healthcare workers in two selected teaching and non-teaching hospitals.Methods: Seventy-five hospital employees participated in this study. To assess sound levels in various hospital departments, a calibrated sound level meter (CEL-231) was employed. Saliva samples were taken to measure cortisol levels during fasting and non-fasting conditions at both morning and evening sessions. Descriptive statistics, independent and paired t-tests, and analysis of variance (ANOVA) were utilized for data analysis.Results: The study findings revealed that in both Amir al-Mu'minin (A) and Abuzar hospitals in Ahvaz, the mean and standard deviation of sound pressure levels were 6 ± 3.75 and 5 ± 9.76 decibels, respectively. Salivary cortisol concentrations indicated a significant difference between normal feeding and fasting conditions (P < 0.05). A notable difference in salivary cortisol levels was found between the non-exposure-to-noise condition and the hunger and noise exposure condition (P < 0.05). Regarding the influence of hospital environment on cortisol levels, there was a significant difference in cortisol secretion between the staff of the two hospitals (P = 0.00).Conclusion: The findings of this study suggest that the average sound pressure level in workplace environments significantly affects workers' salivary cortisol concentrations. Additionally, the combination of noise exposure and fasting exerts a synergistic effect on salivary cortisol levels.