Introduction and Objective: The overuse and unnecessary consumption of antibiotics have led to resistance in many bacteria, creating a global challenge. This study aims to investigate the antibiotic resistance patterns of microorganisms isolated from various clinical samples in the pediatric ward of Ali Ibn Abi Talib Hospital in Zahedan.Materials and Methods: In this descriptive study, after obtaining approval from the ethics committee, we reviewed 531 pediatric hospitalization records at Ali Ibn Abi Talib Hospital. Only complete files meeting the study's inclusion criteria were analyzed.Results: Among 283 bacteria isolated from urinary samples, the most common were Escherichia coli (64.3%), Klebsiella (14.5%), Staphylococcus (6.4%), Enterobacter (5.7%), and Proteus (5.7%). Of 180 bacterial isolates from blood cultures, the most prevalent were Staphylococcus epidermidis (51.11%), Staphylococcus aureus (10%), Acinetobacter (10%), and Escherichia coli (9.44%). Among wound infections, Pseudomonas aeruginosa accounted for 31.7%, followed by Staphylococcus aureus (16.8%), Acinetobacter (10.17%), and Klebsiella (9.59%).Discussion and Conclusion: Overall, the study revealed the highest sensitivity to imipenem and ciprofloxacin, whereas ampicillin exhibited the highest bacterial resistance.